RBL2 (Retinoblastoma-like Protein 2, Retinoblastoma-related Protein 2, RBR-2, PRB2, 130kD Retinoblastoma-associated Protein, p130, RB2)

Two retinoblastoma-related proteins, p107 and pRb2/p130 are structurally and functionally similar to the product of the retinoblastoma gene (pRb/p105). Like pRb they bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Both proteins play a fundamental role in growth control. The highest percentage of undetectable levels, tightest inverse correlation with histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2/p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2hrs at RT\nImmunoprecipitation: 2ug/mg protein lysate\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT\nStaining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTonsil, A427 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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