RBP4, Mouse, BioAssay(TM) ELISA Kit (Retinol-binding Protein 4, Plasma Retinol-binding Protein, PRBP, RBP)

Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of mouse RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (mouse/rat) ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat RBP4 in serum, urine and cell culture supernatant. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat RBP4. It does not cross-react with human RBP4, mouse adiponectin, mouse resistin, mouse Nampt, rat adiponectin, rat resistin, rat Nampt, mouse RELM-a, mouse RELM-b, human leptin, rat RELM-a, mouse 41BBL, mouse CD137, mouse Tim3, human TNF-a. The assay range is 0.188-12ng RBP4/ml and a detection limit of 60pg/ml (based on adding 2 standard deviations to the mean value of the (50) zero standards).\n\nSample Type:\nSerum, urine, or cell culture supernatant.\n\nIntended Use:\nFor the in vitro quantitative determination of mouse or rat RBP4 in serum, urine and cell culture supernatant.\n\nSensitivity:\n60pg/ml \n\nRange:\n0.188-12ng RBP4/ml\n\nSpecificity:\nMammalian\n\nTest Principle:\nThis assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat RBP4. It does not cross-react with human RBP4, mouse adiponectin, mouse resistin, mouse Nampt, rat adiponectin, rat resistin, rat Nampt, mouse RELM-a, mouse RELM-b, human leptin, rat RELM-a, mouse 41BBL, mouse CD137, mouse Tim3, human TNF-a.\n\nKit Components:\nPre-coated Microtiter Plate: 12x8 well strips\nWash Buffer (10X): 1x50ml\nDiluent (5X):1x50ml\nDetection Antibody: 1x12ml\nDetector 100X (Hrp conjugated anti-IgG): 1x150ul\nMouse RBP4 Standard (lyophilized, 24ng): 1 vial\nMouse RBP4 QC Sample (lyophilized): 1 vial\nSubstrate Solution I (TMB): 1x6ml\nSubstrate Solution II (Peroxidase): 1x6ml\nStop Solution: 1x12ml\nPlate Sealers: 3 each\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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