Receptor, soluble (sLeptin, sLR)

Leptin is a cytokine that is primarily expressed by adipose tissue. Leptin controls food intake by its interaction with the leptin receptor in the brain. Leptin action is mediated and controlled by the leptin receptor, a class I type cytokine receptor. sLR is generated by proteolytic cleavage of membrane-anchored receptors. This indicates that the leptin receptor might have other functions besides transduction. Binding of leptin with sLR increases the bioavailability of leptin in plasma, but also decreases the binding of leptin to membrane bound leptin receptors. For example, when comparing obese and lean individuals, plasma levels of sLR are significantly decreased whereas leptin levels are significantly increased.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:50\nImmunohistochemistry (frozen and paraffin): 1:50\nOptimal dilution to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L1670-08W

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. van Dielen, F et al; Leptin and soluble leptin receptor levels in obese and weight-losing individuals. J Clin Endocrinol Metab 2002, 87: 1708

Additional Info

Recognizes human soluble leptin receptor (sLR) in plasma. It specifically reacts with sLR with a molecular mass of 180kD. The 2F1 antibody can be used to measure in both free sLR and sLR bound to leptin in plasma.