The 706-Cre plasmid is designed for use in Cre-mediated genomic manipulations. The plasmid has a pSC101 origin which maintains low copy and replicates at 30Degree C. The plasmids will not propagate and will get lost when incubated at 37Degree C.
The expression of the Cre-recombinase is driven by the thermosensitive promoter cI578 (?PR promoter). Therefore, the expression of Cre is repressed at 30Degree C and induced between 37-42Degree C. The plasmid carries a tetracycline resistance.
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