Resistin (ADSF, Adipose Tissue-Specific Secretory Factor, FIZZ3, Found in Inflammatory Zone)

Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic drugs, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Resistin, specifically produced and secreted by adipocyte, is present at elevated levels in the blood of obese animals, and is down regulated by fasting and anti-diabetic drugs. Antibody to resistin stimulated glucose uptake and improved insulin sensitivity in obese mice. Some other protein related to resistin has been called resistin-related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allergy and inflammation.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA (Direct and indirect): 1:2000-1:10,000.\nWestern Blot: 1:2000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween-20 with 5% skim milk. Suggested incubation time is 1 hour at RT.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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