Resistin-like Molecule Protein, beta, Human (RELMb)

Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic drugs, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Resistin, specifically produced and secreted by adipocyte, is present at elevated levels in the blood of obese animals, and is down regulated by fasting and anti-diabetic drugs. Antibody to resistin stimulated glucose uptake and improved insulin sensitivity in obese mice. Some other protein related to resistin has been called resistin-related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allergy and inflammation.\nRELM-beta/FIZZ2 (prepeptides: mouse 105 aa; human 11 aa ~45-65% identity with FIZZ1 and 2) is highly related to resistin at the C-terminus region. Its expression is most abundant in colon (proximal and distal colon) but not in white adipose tissue. In mouse, RELM-beta is also detected at low levels in cecum and ileum. In min mouse (mouse model for human familial adenomatosus polyposis), RELM-beta expression was markedly increased in tumors immediately adjacent to normal tissue.\nThe purified protein is a disulfide linked homodimer of ~19kD consisting of two 89aa chains.\n\nApplications:\nSuitable for use as positive control in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:\n10ul per lane. Heat once prior to loading on gels.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1588

Size

50ul

Applications

ELISA, WB

Form

Supplied as a liquid in SDS-PAGE sample buffer (reduced)

Purity

> 98%

References

(1) Steppan CM et al (2001) Nature 409, 307; Steppan CM et al (2001) PNAS 98, 502; Holcomb IN et al (2000) EMBO J. 19, 4046-4055; Kim K-H et al (2001) J. Biol. Chem. 276, 11252-11256