Resistin (RETN, RETN1, RSTN, Adipose Tissue-Specific Secretory Factor, ADSF, FIZZ3, Cysteine-rich Secreted Protein A12-alpha-like 2, Cysteine-Rich Secreted Protein FIZZ3, C/EBP-epsilon-Regulated Myeloid-Specific Secreted Cysteine-Rich Protein, HXCP1, RSTN

RELM:FIZZ family of proteins are Cysteine-rich proteins consist of a highly conserved C-terminal cysteine rich domain and play a vital role in regulation of adipogenesis and insulin resistance. Resistin, a human homolog of mouse resistin, is a novel member of this family. It is a 12kD adipokine which is produced by both brown and white adipose tissue and is mainly secreted by adipocytes. Its levels are down-regulated by insulin and have been shown to be elevated during Type 2 diabetes, indicating a critical role in regulating adipogenesis and insulin resistance. It promotes aortic smooth muscle cell proliferation through activation of ERK1:2 and PI3:Akt signaling pathways. It is expressed in macrophages and functions as a pro-inflammatory cytokine by up-regulating IL-6 and activating NF-kappaB signaling pathway. Its expression is regulated by numerous factors which include PPAR-alpha, Insulin, Growth and gonadal hormones, TNF-alpha, Endothelin-1 and certain pro-inflammatory cytokines like IL-6.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1585-02L

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Steppan et al. (2001) Nature 409: 307-312 2. Banerjee RR et.al J. Biol Chem., 276, 25970-25973 (2001) 3. HB Hartman et.al J. Biol. Chem., 277 (22); 19754-19761 (May 31, 2002) 4. Patel L et.al Biochem Biophys Res Commun. 300(2):472-6 (2003 Jan 10) 5. Bokarewa M et.al J Immunol. 174(9):5789-95 (2005 May 1)

Additional Info

Recognizes human Resistin.

Alternative Names

Cysteine-Rich Secreted Protein A12-alpha-Like 2, Found in Inflammatory Zone, MGC126603, MGC126609