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RGS3 (Regulator of G Protein Signaling 3, RGP3)

Cat no: R1995-15D

RGS3 (Regulator of G Protein Signaling 3, RGP3)

RGS3 (regulator of G protein signaling 3) is a 519-residue protein that is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase-activating protein (GAP). RGS3 negatively regulates the activation of Gia and Gaq. A truncated RGS3 (RGS3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS3T may be localized in the nucleus and RGS3 is found in cytoplasm. Translocation to membrane is important for the RGS proteins to interact with heterotrimeric G proteins.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1995-15D

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human RGS3.

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