RGS4 (Regulator of G Protein Signaling 4, Regulator of G Protein Signalling 4, RGS-4, MGC2124, MGC60244, RGP4, SCZD9)

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120 amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat RGS4. This protein negatively regulates signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 1:200\nImmunohistochemistry: 1:200\nWestern Blot: 1:2000. Predicted molecular weight: 35kD.\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Cytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1995-22A

Size

50ug

Applications

ICC, IHC, WB

Hosts

Ch/Bird

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS containing 0.02% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgY

References

Berman DM et al. GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein alpha subunits. Cell 86:445-52 (1996). <PUBMED:8756726>

Additional Info

Recognizes RGS4. Species Crossreactivity: human, mouse and rat