A cell-permeable isoquinolinesulfonamide compound that acts as a highly specific, reversible, potent, and ATP-competitive inhibitor of G-protein Rho-associated kinase (ROCK; Ki=1.6nM). Inhibits other serine/threonine kinases only at higher concentrations (Ki=630nM for PKA, 9.27 uM for PKC, and 10.1uM for MLCK). Shown to selectively block lysophosphatidic acid-induced, but not PDBu-induced, phosphorylation of myristoylated alanine-rich C kinase substrate (MARCKS) in NT-2 cells (IC50=2.5uM). Reported to be a more potent and selective inhibitor than Y-27632.
Solubility:
H2O (5mg/ml) or Methanol (5mg/ml)
Molecular Weight:
~392.3
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.