Ribosomal Protein P0, Recombinant, Human (RPP0)

The ribosomal phosphoproteins(P protein antigens) are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35kD, P1 (19 kD) and P2 (17 kD). Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies.\n\nDescription: \nRecombinant Human Ribosomal Phosphoprotein P0 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35,096 dalton. Recombinant Human Ribosomal Phosphoprotein P0 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.\n\nImmunological Functions:\n1. Binds IgG-type human auto-antibodies.\n2. Standard ELISA test (checker-board analysis of positive/negative sera panels.\n\nApplications: \nWestern-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease).\n\nCoating Concentration:\n0.3-0.7ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

R2031-28R

Size

5ug

Form

Supplied as a liquid in 20mM HEPES buffer, pH 7.5, 0.01mM EDTA. 0.02% SDS.

Purity

(same/more than) 80% (SDS-PAGE)

References

1. Up-regulation of acidic ribosomal phosphoprotein P0 in response to freezing or anoxia in the freeze tolerant wood frog, Rana sylvatica.\n\nCryobiology 200 5 Feb;50(1):71-82\n\n2. Baculovirus-mediated expression and isolation of human ribosomal phosphoprotein P0 carrying a GST-tag in a functional state.\n\nBiochem Biophys Res Commun 2004 Sep 24;322(3):814-9\n\n3. Identification of a hypothetical membrane protein interactor of ribosomal phosphoprotein P0.\n\nJ Biosci 2004 Mar;29(1):33-43\n\n4. Molecular cloning and sequence analysis of canine acidic ribosomal phosphoprotein P0 cDNA.\n\nDNA Seq 2000;11(3-4):321-6\n\n5. Antibodies against ribosomal phosphoprotein P0 of Plasmodium falciparum protect m ice against challenge with Plasmodium yoelii.\n\nInfect Immun 2000 Jul;68(7):4312-8\n\n6. Cloning of a ribosomal phosphoprotein P0 gene homologue from Plasmodium falciparum.\n\nMol Biochem Parasitol 1996 Nov 12;82(1):117-20