RING Finger Protein 111 (RNF111, E3 Ubiquitin-protein Ligase Arkadia, ARK)

Applications:\nSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nMSQWTPEYNELYTLKVDMKSEIPSDAPKTQESLKGILLHPEPIGAAKSFPAGVEMINSKVGNEFSHLCDDSQKQEKEMNGNQQEQEKSLVVRKKRKSQQAGPSYVQNC\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

132631

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. RB1CC1 positively regulates transforming growth factor-{beta} signaling through the modulation of Arkadia E3 ubiquitin ligase activity. Koinuma D, Shinozaki M, Nagano Y, Ikushima H, Horiguchi K, Goto K, Chano T, Saitoh M, Imamura T, Miyazono K, Miyazawa K.J Biol Chem. 2011 Jul 27. 2. Efficient TGF-beta/SMAD signaling in human melanoma cells associated with high c-SKI/SnoN expression. Javelaud D, van Kempen L, Alexaki VI, Le Scolan E, Luo K, Mauviel A.Mol Cancer. 2011 Jan 6;10(1):2. 3. Context-dependent regulation of the expression of c-Ski protein by Arkadia in human cancer cells. Nagano Y, Koinuma D, Miyazawa K, Miyazono K.J Biochem. 2010 Apr;147(4):545-54. Epub 2009 Dec 2. 4. Overexpression of snon/skil, amplified at the 3q26.2 locus, in ovarian cancers: a role in ovarian pathogenesis. Nanjundan M, Cheng KW, Zhang F, Lahad J, Kuo WL, Schmandt R, Smith-McCune K, Fishman D, Gray JW, Mills GB.Mol Oncol. 2008 Aug;2(2):164-81. Epub 2008 May 10. 5. Arkadia activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation. Levy L, Howell M, Das D, Harkin S, Episkopou V, Hill CS.Mol Cell Biol. 2007 Sep;27(17):6068-83. Epub 2007 Jun 25.

Additional Info

Recognizes human RNF111.

Alternative Names

EC=6.3.2.-, DKFZp313E0731, DKFZp686H1966, DKFZp761D081, FLJ38008