Blebbistatin blocked myosin II-dependent cell processes. It blocks cell blebbing rapidly and reversibly.
As part of a defined medium for growth of human Pluripotent stem (hPS) cells, Blebbistatin acts as an inhibitor of nonmuscle myosin II (NMII) and enhances the survival of hPS cells.
Preventing cell death of Human pluripotent stem (hPS) cells, such as human embryonic stem (hES) and induced pluripotent stem (hiPS) cells, has been a stumbling block to avenues of investigation of with these cell types. The most current culture methods for growing hPS cells require animal-derived materials like Matrigel, for coating the culture surfaces, without which hPS cells cannot adhere to the culture plate. The drawback in using these feeder cells is they can potentially contaminate hPS cells by introducing viruses and other unknown pathogens.
The active enantiomer of Blebbistatin (so named because of its ability to block cell blebbing) is the (S)-(-)-Blebbistatin form. United States Biological offers the racemic mixture of Blebbistatin, along with the active (S)-(-) form and the inactive (R)-(+) form (which can be used as a negative control).
Source: Synthetic
Melting Point: 206-213 degrees C
Solubility: Chloroform (heated), Dichloromethane (sonication)
Method for Determining Identity: Proton NMR Spectroscopic and Mass Spectrometric Analysis
TLC Conditions:
SiO2: Dichloromethane: Isopropyl Alcohol; 9ml: 1ml; Visualized with UV and naked eye; Single Spot; Rf=0.5.
Specific Rotation: -496 (c=0.2, Dichloromethane)
Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20 degrees C. Light sensititve. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
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