S-Glutathionylated Protein Detection Kit

Glutathione (GSH) is involved in maintenance of protein sulfhydryl reduction status. PSSG may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. Cayman's PSSG provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis. This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any PSSG adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired.

Prices direct from Cayman Chemical Company

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

10010721

Form

1 ea

P Type

Assay Kits

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Glutathione (GSH) is a tripeptide ((gamma)-glutamylcysteinylglycine) widely distributed in both plants and animals. GSH is involved in maintenance of protein sulfhydryl reduction status. The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver. Mixed protein glutathionyl disulfides are a post translational protein modification of growing interest. Protein S-glutathionylation (PSSG) may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. PSSG detection methods can employ GSH adduct antibodies, GSH derivatives, and differential labeling systems based on the