S6 Ribosomal Protein, phosphorylated (Ser235/236) (Alexa Fluor(R) 647)

To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).\n\nApplications: \nSuitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry 1:10\nImmunofluorescence: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nStore at 2-8 degrees C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. AlexaFluor conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

R2031-29D

Size

500ul

Applications

FC, IF

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA.

P Type

Mab

Purity

As reported

Isotype

IgG

References

(1) Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100

Additional Info

Detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser235 and 236. The antibody was conjugated to Alexa Fluor(R) 647 under optimal conditions with an F/P ratio of 2-5. The Alexa Fluor(R) 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Unconjugated antibody reacts with human, monkey, mouse and rat Phospho-S6 Ribosomal protein and it is expected that Phospho-S6 Ribosomal Protein (Ser235/236) Rabbit mAb (Alexa Fluor(R) 647 conjugate) will also recognize Phospho-S6 in these species.