sCD100, Recombinant, HumansCD100, Recombinant, Human (Semaphorin-4D, BB18, A8, GR3)

The Semphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150kD transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC cooperation. The CD100 precursor contains 862aa, including a 21aa signal sequence, a 713aa extracellular domain, a 21aa transmembrane sequence, and a 107aa cytoplasmic region. The extracellular sequence contains several structural features, including a 479aa sema domain, a 79aa Ig-like sequence, and a 52aa Plexin-type repeat Recombinant soluble CD100 is a 78.9kD protein comprising the extracellular domain of CD100 (711aa). SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer. Source: Recombinant protein corresponding to human sCD100, expressed in CHO cells. Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDVFVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKYM QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLLSSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHGGTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN LSEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSVVQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TGTSCEPKIV INTVPQLHSE KTMYLKSSDN R Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.