SCF, Recombinant, Human, CF (Kit Ligand, Mast Cell Growth Factor, MGF, Stem Cell Factor, c-Kit Ligand, KITLG)

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40kD type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189aa extracellular domain (ECD), a 23aa transmembrane segment, and a 36aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25kD soluble molecule which is comparable to the only formproduced by Steel-dickie mutant mice. An alternatively spliced isoform of human SCF exists that lacks 28aa that encompass the primary proteolytic recognition site. Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%-87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.\n\nSource:\nRecombinant corresponding to aa26-189 from human HEK293.\n\nMolecular Weight:\n~18.5kD\n\nEndotoxin: ~1EU/1ug (LAL)\n\nBiological Activity:\nMeasured in a cell proliferation assay using TF1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 15ng/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7973-01B

Size

10ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 100ug/ml.

Purity

~95% (SDS-PAGE)

References

1. Ashman, L.K., 1999, Int. J. Biochem. Cell Biol. 31:1037. 2. Sette, C. et al., 2000, Int. J. Dev. Biol. 44:599. 3. Yoshida, H. et al., 2001, J. Invest. Dermatol. Symp. Proc. 6:1.4. Erlandsson, A. et al., 2004, Exp. Cell Res. 301:201. 5. Kapur, R. et al., 2002, Blood 100:1287.6. Wang, C.H. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27:540. 7. Bashamboo, A. et al., 2006, J. Cell Sci. 119:3039. 8. Reber, L. et al., 2006, Eur. J. Pharmacol. 533:327. 9. Martin, F.H. et al., 1990, Cell 63:203.10. Arakawa, T. et al., 1991, J. Biol. Chem. 266:18942. 11. Majumdar, M.K. et al., 1994, J. Biol. Chem. 269:1237.12. Brannan, C.I. et al., 1991, Proc. Natl. Acad. Sci. USA 88:4671. 13. 13. Anderson, D.M. et al., 1991, Cell Growth Differ. 2:373. 14. 14. Lemmon, M.A. et al., 1997, J. Biol. Chem. 272:6311.15. 15. Kanellakis, P. et al., 2006, Cardiovasc. Res. 70:117.