SCF (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, Steel Factor, SF)

SCF is a type I transmembrane protein and hematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor. Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of hematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. Studies have shown involvement of the association between SCF and c-Kit with the progression and invasiveness of tumors, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia.\n\nApplications:\nSuitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nELISA: 0.5ug/ml\nWestern Blot: 0.1-0.2ug/ml\nFunctional Assays: 0.008-0.012ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0400-07V

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile 40-50% glycerol, ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. O' Laughlin-Bunner, B. et al. (2001) Lyn is required for normal stem cell-factor induced proliferation and chemotaxis of primary hematopoietic cells. BLOOD. 98: 343-350. 2. Ronnstrand, L. (2004) Signal transduction via the stem cell factor receptor/c-Kit. Mol. Life Sci. 61: 2535-2548.

Additional Info

Recognizes human stem cell factor (SCF)