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Sec61a

Cat no: S0580

Sec61a

SEC61 alpha subunit isoform 1 (SEC61a1), a member of the SECY/SEC61-alpha family. SEC61a1 is an integral membrane protein which is localized to the endoplasmic reticulum. The protein appears to play a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:2000 detects Sec61a from a rat brain microsomal preparation. Note: DO NOT BOIL microsomes. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with S0580 (1:2500). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nImmunoprecipitation: Reported to immunoprecipitate Sec61a crosslinked to nascent polypeptide chains.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0580

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Can

Form

Supplied as a liquid, 0.05% sodium azide, 30% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Hanein, D., et al., Cell 87: 721-732 (1996) Abstract (quantitative immunoblotting). 2. Jungnickle, B. and T.A. Rapoport, Cell 82: 261

Additional Info

Recognizes human Sec61a at ~40kD. Species Crossreactivity: canine. Species Sequence Homology: mouse and rat, 100%.

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