Semaphorin 3E (Semaphorin-3E, SEMA3E, KIAA0331, SEMAH)

Semaphorin 3E is one of six Class 3 (secreted) semaphorins which in the human share 40-50% aa identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development. Sema3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels. Crystal structures of semaphorins reveal that the 500aa N-terminal Sema domain forms a seven-blade b-propeller similar to that found in integrin molecules; 14 conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure. C-terminal to the Sema domain, Sema3E has a consensus sequence for furin cleavage which, when used, creates a 61kD form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential. Further C-terminal are a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and a basic domain containing another furin site. Dimerization and cleavage at the C-terminal site are required for repulsing activity of class 3 semaphorins. Human Sema3E shares 90%, 85% and 57% aa identity with mouse, cow and dog Sema3E, respectively. Like other semaphorins, Sema3E signaling is transduced by a transmembrane Plexin dimer, which also has a Sema domain and is coupled to kinase pathways. Unlike other Class 3 semaphorins, Sema3E binds directly to its plexin and does not require interaction with a neuropilin for activity. Genetic disruption of either Sema3E or PlexinD1 creates mouse mutants with excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml\nImmunohistochemistry: 5-15ug/ml\nImmunocytochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0668-50H

Size

100ug

Applications

ICC, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Eichmann, A. et al. (2005) Genes Dev. 19:1013. 2.Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767. 3.Gu, C. et al. (2005) Science 307:265. 4.Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669. 5.Christensen, C. et al. (1998) Cancer Res. 58:1238. 6.Christensen, C. et al. (2005) Cancer Res. 65:6167. 7.Adams, R. H. et al. (1997) EMBO J. 16:6077. 8.Gitler, A. D. et al. (2004) Developmental Cell 7:107.

Additional Info

Recognizes human Semaphorin 3E. Species sequence homology: Recombinant mouse Semaphorin 3E (70%), mSemaphorin 3C and rmSemaphorin 3F (5%).