Semaphorin 3F (Biotin)

The semaphorins make up a family of inhibitory axon guidance molecules that include secreted, transmembrane, and GPI anchored extracellular molecules. Via their interaction with Plexin/Neuropilin receptor complexes, the semaphorins are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3F belongs to the class 3 secreted group of molecules and shows ubiquitous expression in a range of neuronal and non-neuronal tissues including brain, mammary gland, liver, kidney, lung, spleen, muscle and heart. The role of the semaphorins in non-neuronal tissues is poorly characterised.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.1-0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0668-51

Size

50ug

Applications

WB

Hosts

Sheep

Reactivities

Hum, Mouse

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Semaphorin 3F. Species Crossreactivity: mouse.