Sentrin 1 (SUMO-1, SMT3C)

Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (sumoylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process and is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin regulates predominantly degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and I B- to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP and regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer's disease (8-11).\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot 1:1000\nImmunoprecipitation 1:50\nImmunohistochemistry (paraffin): 1:50. Antigen retrieval: citrate method. Antibody diluent: TBST/5% normal goat serum\nImmunocytochemistry/Immunofluorescence: 1:100\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0750-01K

Size

100ul

Applications

ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

(1) Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem. Sci. 28, 321

Additional Info

Detects recombinant SUMO-1 and and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD). Species Crossreactivity: Human, mouse and rat.