Serine, Phosphorylated (Biotin)

Protein phosphorylation and dephosphorylation reactions is a key postranslational event in the modification of protein functions. Protein phosphorylation occurs at tyrosine, serine, or threonine (p-tyr, p-ser, or p-thr) residues. Many different mitogenic systems, such as the EGF, PDGF, and insulin receptor systems contain tyr/ser/thr kinase domains which autophosphorylate specific tyr, ser or thr residues upon binding of their ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated proteins. An understanding of transformation by oncogenes and mitogenic processes of growth factors requires a delineation of all potential partners involved in phosphorylation cascade. The availability of antibodies specific for p-tyr, p-thr or p-thr residues has greatly advanced the studies on role of phosphorylated proteins.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:5000-1:30,000\nWestern Blot: 1:2000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1000-75A

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH7.4, 0.2% BSA, 0.05% sodium azide. Labeled with Biotin. F/P ratio ~10-20:1.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Hunter, T. (1985) Annu. Rev. Biochem., 54, 897. 2. Heffetz, D. (1991) Enzymol., 201, 44. 3. Alexander, D.et al (1989) Immunol. Today, 10, 200. 4. Levine, L., et al., (1989) J. Immunol. Methods, 124, 239.

Additional Info

Recognizes phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated serine, phosphorylated tyrosine or threonine, AMP, or ATP.