Serotonin Receptor 1D (5-Hydroxytryptamine (Serotonin) Receptor 1D, 5-Hydroxytryptamine Receptor 1D, 5HT1D, 5-HT1D, 5-HT-1D, Htr1d)

Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1,2). The 5-HT1D (formerly known as 5-HT1Da) receptor has 63% overall structural homology with the 5-HT1B receptor and a 77% aa sequence homology in the seven transmembrane domains (3). In situ hybridization studies have detected highest level of mRNA in primary olfactory cortex, accumbens nucleus, caudate putamen, lateral mammilary nucleus and medial vestibular nucleus (4). The presence of relatively high levels of 5-HT1D mRNA in the locus coeruleus suggest that, when expressed on nerve terminals, this receptor could modulate the release of catecholamines. The 5-HT1D receptor may function as an autoreceptor. Like 5-HT1B receptors, 5-HT1D receptors also appear to act as heteroreceptors. Acting on the 5-HT1D receptors, 5-HT appears to inhibit release of glutamate from rat cerebellar synaptosomes and acetylcholine from guinea pig hippocampal synaptosomes (5). It has been suggested that neurogenic inflammation and nociceptive activity within trigemino- vascular afferents. Endothelium-dependent relaxation in the pig coronary artery has been claimed to be mediated by 5-HT1D receptors (6). Like other members of the 5-HT1 family, 5-HT1D receptors are negatively coupled to adenylate cyclase.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman, mouse, or rat brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1001-15D2

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Petrouka, SJ. Trends Neurosci. 11: 486-500 (1998). 2. Lauder, JM. Trends Neurosci. 16: 233-240 (1993). 3. Weinshank, RL., Zgombick, JM., Macchi, MJ., Branchek, TA., Hartig, PR. Proc. Acad. Natl. Sci. USA 89: 3630-3633 (1992). 4. Gerhardt, CC. And van Heerikhuizen, H. Eur. J. Pharmacol. 334: 1-23 (1997). 5. Leonhardt, S., Herrick-Davis, K., Titeler, M. Proc. Natl. Acad. Sci. USA 89: 5517-5521 (1989). 6. Miller, KJ. And Teitker, M. Neurosci. Lett. 136: 223-226 (1992). 7. Hamblin,M.W., McGuffin,R.W., Metcalf,M.A., Dorsa, et al. Mol. Cell. Neurosci. 3, 578-587 (1992).

Additional Info

Recognizes rat Serotonin Receptor 5-HT1D (5-HT1DR). Species Crossreactivity: human and mouse.

Alternative Names

5-Hydroxytryptamine 1D Receptor, G Protein Coupled Receptor 14, GPCR14, HT1DA, HTR1DA, HTRL, RDC4