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Serotonin Transporter (SERT, 5HT, 5-hydroxytryptamine)

Cat no: S1001-25B

Serotonin Transporter (SERT, 5HT, 5-hydroxytryptamine)

Serotonin (5-hydroxytryptamine), MW 176, is a catecholamine , a monoamine neurotransmitter, found in abundance in the central nervous system, platelets, brain and spinal cord as well as secreted by enterochromaffin cells (EC or Kulchitsky cells, a type of enteroendocrine cell occurring in the epithelia lining the lumen of the gastrointestinal tract) in the stomach, intestines, appendix and in endocrine cells along the gut and in carcinoid tumors. Platelets and mast cells are rich in serotonin. Serotonin plays important physiological functions at the intestinal level. Human intestinal epithelial Caco-2 cellls functionally express SERT both at their apical and basolateral cell membranes. It is a 630aa residue in human (chromosome 17q11), rat and mouse.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n \nRecommended Dilution: \nWestern Blot: 1:1000\nImmunohistochemistry (Cy3 technique): 1:800-1:1000 in PBS, 0.3% Triton X-100.\nImmunohistochemistry (Biotein/avidin-HRP): 1:10,000- 1:15,000 in PBS, 0.3% Triton X-100 .\nOptimal dilutions to be determined by the researcher.\n\nPositive Control Tissue:\nRat raphe nuclei, hypothalamus, cortex and spinal cord\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1001-25B

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder with sodium azide. Reconstitute with 100ul sterile ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Recognizes rat Serotonin Transporter.

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