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SET07 (Histone H4-K20 Methyltransferase, H4-K20-HMTase, SET Domain-containing Protein 8, PR/SET Domain-containing Protein 07, PR/SET07, PR-Set7, KMT5A, Histone-lysine N-methyltransferase SETD8, H4-K20-HMTase SETD8, Lysine N-methyltransferase 5A, PRSET7, S

Cat no: S1012-28E-Biotin

SET07 (Histone H4-K20 Methyltransferase, H4-K20-HMTase, SET Domain-containing Protein 8, PR/SET Domain-containing Protein 07, PR/SET07, PR-Set7, KMT5A, Histone-lysine N-methyltransferase SETD8, H4-K20-HMTase SETD8, Lysine N-methyltransferase 5A, PRSET7, S

SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity. \n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1012-28E-Biotin

Size

200ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Nishioka, K., et al., Mol. Cell 9(6):1201-1213 (2002). 2. Fang, J., et al., Curr. Biol. 12(13):1086-1099 (2002).

Additional Info

Recognizes human SET07.

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