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SET7/9 Methyltransferase Inhibitor Screening Assay Kit

SET7/9 Methyltransferase Inhibitor Screening Assay Kit

Cat no: 700270

Supplier: Cayman Chemical Company
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SET7/9 is an MT that acts on various substrates including histone 3 at lysine residue 4 (H3K4), p53, and the transcription factor TAF 10. Unlike most SET proteins, SET7/9 is exclusively a mono-methylase. Cayman's SET7/9 MT Inhibitor Screening Assay provides a convenient method for screening SET7/9 inhibitors. The transfer of the methyl group from SAM by SET7/9 to the acceptor peptide (TAF 10) generates SAH, which is rapidly converted to urate and H2O2 using an enzyme mixture provided in the kit. A subsequent reaction between H2O2 and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed using an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm.
Catalogue number: 700270
Weight: 0
Form: 96 Well
P type: Assay Kits|Methyltransferase
Shipping temp: -80
Storage temp: -80
Additional info: SET7/9 is an MT that acts on various substrates including histone 3 at lysine residue 4 (H3K4), p53, and the transcription factor TAF 10. In Cayman

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