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Products SET8 Methyltransferase Inhibitor Screening Assay Kit
| Catalogue number: | 700350 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Methyltransferase |
| Shipping temp: | -80 |
| Storage temp: | -80 |
| Additional info: | SET Domain-containing Protein 8 (SET8) is a methyltransferase that selectively mono-methylates histone H4 at lysine residue 20 (H4K20), an event proven to have an important role in chromatin structure and transcriptional activation. Cayman's SET8 Methyltransferase Inhibitor Screening Assay provides a convenient method for screening human SET8 inhibitors. The transfer of the methyl group from SAM by SET8 (provided in the kit) to the acceptor peptide (H4K20) generates S-adenosylhomocysteine, which is rapidly converted to urate and hydrogen peroxide (H2O2) using an enzyme mixture provided in the kit. The H2O2 formed is quantified using 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to produce the highly fluorescent compound resorufin (excitation 530-540 nm; emission 585-595 nm). |
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