SGLT-1 (Sodium Glucose Transporter 1)

The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucoseco transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75kD). Both N and C-termini are predicted to be extracellualr. There is approx 60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL). Detects a band at ~70-77kD.\nELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with control peptide.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nS1010-86E: SGLT-1, Human, Control Peptide (Sodium Glucose Transporter 1)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nAlso Available:\nS1010-86K: SGLT-1 (Sodium Glucose Transporter 1) Pab Rb xHu (Affinity purified antibody).\n\nMouse related products:\nS1010-86A: SGLT-1 (Sodium Glucose Transporter 1) Pab Rb xMo (antiserum)\nS1010-86B: SGLT-1 (Sodium Glucose Transporter 1) Pab Rb xMo (affinity purified antibody)\nS1010-86F: SGLT-1, Mouse (Sodium Glucose Transporter 1) (control peptide)\n\nRat related products:\nS1010-86C: SGLT-1, Rat (Sodium Glucose Transporter 1) (control peptide)\nS1010-86D: SGLT-1 (Sodium Glucose Transporter 1) Pab Rb xRt (antiserum)\nS1010-86G: SGLT-1 (Sodium Glucose Transporter 1) Pab Rb xRt (affinity purified antibody)

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SPECIFICATIONS

Catalog Number

S1010-86H

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Lee, W.S., et al., JBC 269: 12032 (1994). Gene accession # AF163846. 2. Hediger, M.A., et al., Nature 330: 1379-1381 (1987). 3. Sileverman, M., et al., BBA 1153: 43-52 (1993). 4. Pajor, A.M., et al., Am. J. Physiol. 32: R489-R495 (1992). 5. Hirayama, E.A., et al., BBA 1103: 37-44 (1992). 6. Hirayama, E.A., et al., Am. J. Physiol. 261: C296 (1991). 7. Wright, E.M., Am. J. Renal Physiol. 280: F10 (2001).

Additional Info

Recognizes human SGLT-1. No significant sequence homology exists with other SGLTs. Species sequence homology (56%): mouse, rat. For mouse and rat, we recommend S1010-86D or S1010-86G.