Shc, phosphorylated (Tyr317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nWestern Blot: 0.5ug/ml for HRPO/ECL detection. Use Casein/Tween-20 based blocking and Blot incubation buffers.\nELISA: 0.05ug/ml.\nOptimal conditions must be determined individually for each application.\n\nPositive Control Included:\nH2030-03H1: HepG2 Cell Lysate, Vanadate Treated\nAdd 100ul SDS-PAGE sample buffer and incubate at 90 degrees C for 5 minutes. Aliquot and store at -20 degrees C \nWestern Blot: 20ul/lane (mini gel) for HRP/ECL detection.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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