Shc, phosphorylated (Y317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p67). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p67 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p67 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p67 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p67 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span.\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot Analysis: A 1:250-1:1000 dilution detects phosphorylated SHC in RIPA lysates from HFF cells stimulated with 50ng/ml EGF for 10 minutes. Non-stimulated or EGF-stimulated HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with S1010-92, 1:1000 dilution. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1010-92

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.2M Tris-glycine, 150mM sodium chloride, 5mg/ml BSA, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

Additional Info

Recognizes phosphorylated human SHC protein isoforms, Mr 46kD, 52kD and 67kD. In long exposures, additional proteins of Mr 110 and 180 are detected in EGF-treated lysates. Species Crossreactivity: mouse.