Home  >  Products  >  SHC1, phosphorylated (Tyr427) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)
SHC1, phosphorylated (Tyr427) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

SHC1, phosphorylated (Tyr427) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Cat no: S1010-90E

Supplier: United States Biological
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he human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type (1-3). Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span (4-5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: S1010-90E
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Pelicci et al. Cell 70: 93-104, 1992. 2. Ventura, A., Luzi, L., Pacini, S., Baldari, C. T., and Pelicci, P. G. (2002) J. Biol. Chem. 277, 22370-22376. 3. Migliaccio et al. (1997) EMBO J. 16, 706-716 4. Migliaccio et al. Nature 402: 309-313, 1999. 5. Aronheim A, Engelberg D Li N, Al-Alawi N, Schlessinger J and Karin M (1994), Cell, 78, 949
Additional info: Recognizes human SHC1, phosphorylated (pY427). Species crossreactivity: mouse and rat.

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