SHP 2 (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

SHP2 is a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP 2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines. In addition to its role in Gab1-mediated Erk activation, SHP 2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-4ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts. Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng) used for immunoprecipitation; use 1ug/ml for this application.\nImmunoprecipitation: 6ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells. An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells.\nImmunocytochemistry: 10ug/ml detects SHP 2/SHPTP-2 in EGF stimulated A431 cells.\nNeutralization: Does not inhibit phosphotyrosine phosphatase activity. \nOptimal dilutions to be determined by the researcher.\n\nPositive Antigen Control Included: \nA0001-50: Non stimulated A431 cell lysate. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1013-29

Size

400ug

Applications

ICC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Feng, G.S., et al., Science 259: 1607 (1993). 2. Bruhns, P., et al., J. Biol. Chem. 275: 37,357-37,364 (2000). 3. Rivard, N., et al., J. Biol. Chem. 270: 11,017-11,024 (1995).

Additional Info

Recognizes mouse SHP 2/SHPTP-2 at 64kD. Apparent size increases to ~70kD upon tyrosine phosphorylation. Species Crossreactivity: human and rat.