SHP 2 (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:32,000\nWestern Blot: 1-3ug/ml ~70kD band observed in Human Skeletal Muscle lysates and in lysates of cell line HEK293 (calculated MW of 68.0kD according to NP_002825.3). In transfected HEK293 transiently expressing PTPN11 bands of approx. 70kD and 49kD are observed. These bands are not observed in the non-transfected HEK293.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1013-30A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Takagi K, Takagi M, Kanangat S, Warrington KJ, Shigemitsu H, Postlethwaite AE.\nModulation of TNF-alpha gene expression by IFN-gamma and pamidronate in murine macrophages: regulation by STAT1-dependent pathways. J Immunol. 2005 Feb 15;174(4):1801-10

Additional Info

Recognizes human SHP2. Species sequence homology: mouse, rat, bovine.

Alternative Names

protein tyrosine phosphatase-2, MGC14433, protein tyrosine phosphatase, non-receptor type 11, protein-tyrosine phosphatase 2C, Noonan syndrome 1, SH-PTP3, SH-PTP2, PTP-1D, SHP-2, PTP2C, BPTP3, NS1, CFC, protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1), SHP2, PTPN11