SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Applications: \nSuitable for use inFlow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: 1:100-1:200 Use 10ul to label 10e6 cells in 100ul\nImmunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.\nOptimal dilution determined by the researcher\n\nRecommended Secondary Reagents: \nIgG Goat anti mouse IgG:FITC (rat adsorbed)\nIgG Goat anti mouse IgG:RPE (rat adsorbed)\n\nImmunohistochemistry: \nRecommended Secondary Reagents: \nGoat anti mouse IgG:HRP (rat adsorbed)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-87Q

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Robinson, A.P. et al. (1986). Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57: 239-247. 2. Barclay, A.N. (1981). The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42: 593-600. 3. Adams, S. et al. (1998). Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J. Immunol. 161: 1853-1859.

Additional Info

Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. Binds to a different epitope on SIRP than ED9. Does not block the interaction of CD172a-CD47.