SIRT3 (Sirtuin 3, hSIRT3, NAD-dependent Protein Deacetylase Sirtuin-3, Mitochondrial, Regulatory Protein SIR2 Homolog 3, SIR2-like Protein 3, SIR2L3)

The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence, lifespan extension and several age-related diseases. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse Heart Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146664

Size

100ug

Applications

ELISA, WB

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

1. Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature 2006; 444:868-74. 2. Lavu S, Boss O, Elliott PJ, et al. Sirtuins- novel therapeutic targets to treat age-associated diseases. Nat. Rev. Drug. Disc. 2008; 7:841-53. 3. Onyango P, Celic I, McCaffery JM, et al. SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase localized to mitochondria. Proc. Natl. Acad. Sci. USA. 2002; 99:13653-8. 4. Hirschey MD, Shimazu T, Goetzman E, et al. SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature 2010; 464:121-5.

Additional Info

Recognizes human SIRT3. Species Crossreactivity: mouse and rat.