SIRT6 GST, Assay Kit, Chemiluminescent, BioAssay(TM) (NAD-dependent Deacetylase Sirtuin-6, SIR2-like Protein 6, SIR2L6)

The SIRT6 Chemiluminescent Activity Assay kit is designed for screening and profiling Sirtuin6 inhibitors by measuring autoribosylation of GST-SIRT6 fusion protein. The nuclear protein SIRT6 is a weak deacetylase but is endowed with a robust ADP-ribosyltransferase activity. The SIRT6 Chemiluminescent Assay Kit comes in a convenient 96-well format, with Sirtuin assay buffer, activated DNA, and purified SIRT6 enzyme for up to 100 enzyme reactions. The key to the SIRT6 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for SIRT6 reactions. First, GST-SRT6 is coated on a 96-well glutathione-precoated plate. Next, the SIRT6 biotinylated substrate is added to the wells. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.\n\nIntended Use:\nDesigned for screening and profiling Sirtuin 6 inhibitors by measuring autoribosylation of GST-SIRT6 fusion protein.\n\nTest Principle:\nThe key to the SIRT6 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for SIRT6 reactions. First, GST-SRT6 is coated on a 96-well glutathione-precoated plate. Next, the SIRT6 biotinylated substrate is added to the wells. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.\n\nKit Components:\nSirtuin 6 (SIRT6: 1x100ug\n20x Assay mixture containing biotinylated substrate: 1x50ul @5mM\nSirtuin assay buffer (10X): 1x1.5ml\nActivated DNA: 1x500ul\nStreptavidin-HRP: 1x100ul\nHRP chemiluminescent substrate: 2x6ml\n96-well glutathione-precoated plate: 1 plate\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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