Sm (Smith) Antigen (BSA & Azide Free)

The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE. \n\nApplications: \nSuitable for use in Electron Microscopy, Immunofluorescence, Western Blot, Immunohistochemistry and Affinity Purification. Other applications have not been tested.\n\nRecommended Dilutions: \nWestern Blot: 0.5-1.0ug/ml for 2 hours at RT\nImmunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minute at RT. No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLS174T cells, Tonsil and Testis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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