Smac (DIABLO, Direct IAP Binding Protein of Low Pi, Second Mitochondrial Activator of Cell Death)

Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27kD. However, the mature protein purified from HeLa cells is only about 25kD (1). \n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry. \nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-47D

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Du C, et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 102 (1): 33-42 (2000). \n2. Chai J, et al. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature 406 (6798): 855-862 (2000). \n3. Srinivasula SM, et al. Molecular determinants of the Caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway. J. Biol. Chem . Aug 18 [epub ahead of print] (2000). \n\nIMG-248 has been cited in the following publications: \n4. Vucie, D., Deshayes, K., Ackerly, H., Pisabarro, M., Kadkhodayan, S., Fairbrother, W., and Dixit, V. J. Biol. Chem . 277:12275-12279 (2002 ). \n5. Jihua Liu, Qiang Dai, Jun Chen, David Durrant, Angela Freeman, Tong Liu, Douglas Grossman and Ray M. Lee. Phospholipid Scramblase 3 Controls Mitochondrial Structure, Function, and Apoptotic Response. Molecular Cancer Research 1:892-902 (2003). \n6. Nam Jin Yoo, et al. Immunohistochemical analysis of Smac/DIABLO expression in human carcinomas and sarcomas. Apmis (Acta Pathologica, Microbiologica et Immunologica Scandinavica) Volume 111 (3): 382 (2003). \n7. Tong Liu, Brook Brouha, Douglas Grossman.

Additional Info

Recognizes human Smac (Second Mitochondrial Activator of Cell Death, DIABLO)