SMAD 1/5/8/9 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma)

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:2000 \nOptimal dilutions to be determined by the researcher.\n\nPositive control: \nSaos-2 \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-55G1

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Gemma,A., Hagiwara,K., Vincent,F., Ke,Y., Hancock,A.R., Nagashima,M., Bennett,W.P. and Harris,C.C. hSmad5 gene, a human hSmad family member: its full length cDNA genomic structure, promoter region and mutation analysis in human tumors. Oncogene 16 (7), 951-956 (1998)

Additional Info

Recognizes human Smad5. Crossreactivity: Smad1, Smad8, Smad9. Species Crossreactivity: mouse and rat.