Smad1 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), are downstream effectors of TGFb/BMP signaling. Smad1 (MADR1) mediates signals of bone morphogenetic proteins (BMPs) in the cell. BMPs constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. BMP receptors are members of the TGFb family of\nSer/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation and activation of these receptors. Subsequently, they phosphorylate Smad1 at serine 463 and 465 in the carboxyl-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the collaborating Smad4 (DPC4) and are translocated into the nucleus, where the transcription of target genes is stimulated.\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-51E

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Prc

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Reacts with the human, mouse and pig Smad1 proteins. On Western blots, it identifies a single band at ~52kDa. A ~140kD band of unknown origin is seen in SK-OV-3 cell lysates and a ~200kD band of unknown origin is seen in NIH3T3 cell lysates. Reactivity has been confirmed with human HepG2 and SK-OV-3, African Green monkey COS-7, and mouse NIH3T3 cell lysates, as well as with fetal mouse muscle and thymus homogenates.