Smad2, phosphorylated (Ser465, Ser467) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Members of the Smad family of signal trans- duction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphory- late them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini by the receptor kinase (serines 465 and 467 on Smad2; serines 423 and 425 on Smad3) by TbetaR-I. Following phosphorylation, Smad2 and Smad3 form a heteromeric complex with the co-smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-53B

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Lyons, R.M., Moses, H.L., Eur. J. Biochem. 187: 467-473 (1990). 2. Massague, J., Annu. Rev. Cell Biol. 6: 597-641 (1990). 3. Roberts, A.B., Sporn, M.B., Growth Factors 8: 1-9 (1993). 4. Heldin, C.H., et al., Nature 390: 465-471 (1997). 5. Massague, J., Annu. Rev. Biochem. 67: 753-791 (1998). 6. Massague, J., Wotton, D. EMBO J. 19: 1745-1754 (2000). 7. Nakao, A., et al., EMBO J. 16: 5353-5362 (1997). 8. Abdollah, S., et al., J. Biol. Chem. 272: 27678-27685 (1997). 9. Souchelnytskyi, S., et al., J. Biol. Chem. 272: 28107-28115 (1997). 10. de Caestecker, M.P., et al., Genes Dev. 12: 1587-1592 (1998).

Additional Info

Recognizes endogenous levels of human Smad2 only when dually phosphorylated at Ser465 and Ser467. May detect phosphorylated Smad3 at its equivalent site. Does not crossreact with other Smad related proteins. Species Crossreactivity: mouse and rat. Species sequence homology: chicken, Xenopus and zebra fish.