Smad5 (MAD Homolog 5, Mothers Against Decapentaplegic Homolog 5)

Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-b family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.

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SPECIFICATIONS

Catalog Number

137492

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Hogan, B.L. et al. (1996) Genes Dev. 10, 1580-1594. 2. Hoodless, P.A. et al. (1996) Cell 85, 489-500. 3. Klemm, J.D. et al. (1998) Annu. Rev. Immunol. 16, 569-592. 4. Kretzschmar, M. et al. (1997) Genes Dev. 11, 984-995. 5. Whitman, M. (1998) Genes Dev. 12, 2445-2462. 6. Sapkota, G. et al. (2007) Mol Cell 25, 441-54. 7. AlarcOn, C. et al. (2009) Cell 139, 757-69.

Additional Info

Recognizes endogenous levels of total human Smad5. Species Crossreactivity: mouse, rat, monkey.