SMARCA4 (Transcription Activator BRG1, ATP-dependent Helicase SMARCA4, BRG1-associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-beta, SWI/SNF-related Matrix-associated Actin-dependent

Also known as Protein BRG1 and SNF2-beta, among other aliases, SMARCA4 a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acts as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, SMARCA4 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma.\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nImmunocytochemistry: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa nuclear extract \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029611

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Schneppenheim, R., et al. (2010). Am J Hum Genet. 86(2):279-284. 2. Fujimoto, M., et al. (2010). Mol Biol Cell. 21(1):106-116. 3. Zager, R.A., and Johnson, A.C. (2009). Am J Physiol Renal Physiol. 296(5):F1032-1041. 4. Kenneth, N.S., et al. (2009). J Biol Chem. 294(7):4123-4131. 5. Voss, T.C., et al. (2009). J Cell Sci. 122(Pt 3):345-356. 6. Osipovich, O., et al. (2007). Nat Immunol. 8(8):809-816. 7. Dammer, E.B., Leon, A., and Sewer, M.B. (2007). Mol Endocrinol. 21(2):415-438. 8. Ramirez-Carrozzi, V.R., et al. (2006). Genes Dev. 20(3):282-296. 9. Sif, S, et al. (1998). Genes Dev. 12(18):2842- 2851. 10. Schnitzler, G., Sif, S., and Kingston, R.E. (1998). Cell. 94(1):17-27.

Additional Info

Recognizes human SMARCA4. Species Crossreactivity: rat.