Smoothelin

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin comprise a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells as well as skeletal and cardiac muscle, do not contain smoothelin. Confocal scanning laser microscopy of tissue sections show a filamentous organization of smoothelin, colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected, i.e., a 59kD isoform specific for visceral SMC (Smoothelin A) and an isoform with a molecular weight of ~100kD in vascular SMC (Smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22. The monoclonal smoothelin antibody is useful for discriminating fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:100-1:1000\nImmunohistochemistry (Frozen/paraffin): 1:100-1:200, using ABC as detection reagent. For paraffin sections, pretreatment in 10mM citrate buffer, pH 6.0 and heating 3 times for 5 minutes in a microwave is required.\nOptimum dilutions to be determined by researcher.\n\nHybridoma:\nSp2/0-AG14 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-62A

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Can, Ch/Bird, Fel, NHP, Prc

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Van der Loop, F.T.L., et al., J. Cell Biol. 134: 401-411 (1996). 2. Wehrens, X.H.T., et al., FEBS Lett. 405: 315-320 (1997). 3. Van der Loop, F.T.L., et al., Arterioscler. Thromb. Vasc. Biol. 17: 665-671 (1997). 4. Christen, T., et al., Circ. Res. 85: 99-107 (1999). 5. Johansson, B., et al., Histochem. Cell Biol. 112: 291-299 (1999). 6. Johansson, B., et al., Histochem. J. 31: 723-727 (1999). 7. Deruiter, M.C., et al., Dev. Dyn. 221: 460-463 (2001). 8. Council, L., et al., (2009) Modern Pathology 22: 639-50. 9. Coco, D. P., et al., (2009). Am J Surg Pathol 33: 1795-1801.

Additional Info

Recognizes chicken 59kD and 100kD protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. Species Crossreactivity: human, canine, feline, porcine and monkey. Does not react with rat.