Smoothelin

Smoothelin is one of the smooth muscle cell specific proteins such as calponin, alpha smooth muscle cell actin, SM22alpha and smooth muscle myosin heavy chain isoforms. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. It co-localizes with actin stress fibers, this distribution indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Two major isoforms of smoothelin have been reported, a 59kD smoothelin-A in visceral smooth muscle cells and a 110kD smoothelin-B in vascular smooth muscle cells. Both gene products are encoded by a single copy gene which is located on chromosome 22 and regulated by a dual promoter system in a tissue specific manner. The variation in the smoothelin proteins is achieved by alternative splicing in the calponin homology domain. Antibodies to Smoothelin are powerful tool in the evaluation of smooth muscle cell differentiatiation as occurs in atherosclerotic lesions.\n\nApplications:\nCan be used for Western Blot, Flow Cytometry and Immunohistology on frozen sections.\n\nRecommended Dilution:\nWestern Blot: 1:10\nFlow Cytometry: 1:10\nImmunohistology: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-62G

Size

100ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Loop, van der F et al; Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells. J Cell Biol 1996, 34: 401. 2. Eys, van G et al; Smoothelin expression characteristics: development of a smooth muscle cell in vitro system and identification of a vascular variant. Cell Struct Funct 1997, 22: 65. 3. Loop, van der F et al; Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype. Arterioscler Thromb Vasc Biol 1997, 17: 665. 4. Johnson, J et al; Injury induces dedifferentiation of smooth muscle cells and increased matrix-degrading metalloproteinase activity in human saphenous vein. Arterioscler Thromb Vasc Biol 2001, 21: 1146. 5. Rensen, S et al; Expression of the smoothelin gene is mediated by alternative promoters. Cardiovasc Res 2002, 55: 850. 6. Maeng, M et al; Adventitial myofibroblasts play no major role in neointima formation after angioplasty. Scand Cardiovasc J 2003, 37: 34.

Additional Info

Recognizes human Smoothelin A and B..