SMT3 (Small Ubiquitin-related Modifier 3, SUMO-3, SMT3 Homolog 1, SUMO-2, Ubiquitin-like Protein SMT3A, SMT3A, SMT3H1)

SUMOylation is a reversible post-translational modification of numerous cellular proteins mediated by SUMO proteins. SUMOylation regulates various cellular processes which include nuclear transport, chromatin structure, DNA repair, protein stability and apoptosis. SUMO consists of unique, highly variable N-terminal extensions, a conserved Ubiquitin domain and C-terminal diglycine cleavage:attachment site. SUMO-3, a 20kD protein, is a novel SUMO paralogue which is highly homologous to Ubiquitin and is a human homolog of S.cerevisiae SMT3. It has the ability to form poly (SUMO) chains along with SUMO-2. Localized throughout the nucleoplasm, it modifies various proteins including Topoisomerase II, C:EBPbeta, and PML. SUMO-3 along with SUMO-1 mediates PIASy-enhanced modification of C:EBPalpha and inhibits transcriptional synergy. SUMO3 might be involved in structure and function of eukaryotic kinetochore.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8026-20F

Size

100ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium. Functional annotation of a full-length mouse cDNA collection Nature 409, 685-690 (2001) 2. Mannen,H., Tsoi,S.C., Krushkal,J.S., Li,W.H. and Li,S.S. The cDNA cloning and molecular evolution of reptile and pigeon lactate dehydrogenase isozymes Mol. Biol. Evol. 14 (11), 1081-1087 (1997) 3. Saitoh et.al J Biol Chem, 275 (9), 6252-6258 (March 3, 2000) 4. Subramanian et.al J. Biol. Chem., 278 (11); 9134-9141 (March 14, 2003) 5. Gill G GENES & DEVELOPMENT 18:2046-2059 (2004).

Additional Info

Recognizes human SMT3. Species sequence homology: bovine, hamster, mouse, porcine, rat.