SNAP23 (Synaptosomal-associated Protein 23, Synaptosomal Associated Protein 23kDa, SNAP-23, SNAP23A, SNAP23B, HsT17016, Synaptosomal Associated Protein, Vesicle Membrane Fusion Protein SNAP23, Vesicle Membrane Fusion Protein SNAP-23)

Supplier:

United States Biological

Cat no: S1014-68B

SNAP23 (Synaptosomal-associated Protein 23, Synaptosomal Associated Protein 23kDa, SNAP-23, SNAP23A, SNAP23B, HsT17016, Synaptosomal Associated Protein, Vesicle Membrane Fusion Protein SNAP23, Vesicle Membrane Fusion Protein SNAP-23)

SNAP23 (Synaptosomal associated protein 23; also Syndet) is a membrane-associated 23-29kD member of the SNAP25 family of proteins. It is expressed in multiple cell types, including neutrophils, platelets, mast cells and adipocytes. SNAP23 is involved in vesicle exocytosis. It has been proposed that SNAP23 can associate with both vesicle and target (cell) membranes via a lipid modification. As a v-SNARE, it may interact with syntaxin-6 at the cell membrane. As a t-SNARE, in conjunction with syntaxin-4, it likely interacts with VAMP-2 and -8 on the vesicle membrane. In either case, this approximates two membranes, which subsequently fuse to create a pore. Human SNAP23 is 211aa in length. It contains two t-SNARE coiled-coil homology domains (aa14-76 and 148-207). Palmitoylation occurs between (aa80-87), while phosphorylation occurs on multiple Ser/Thr residues. There is one splice variant that shows a deletion of (aa90-142). Over (aa146-211), human and mouse SNAP23 share 85% aa identity.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1.0ug/ml\nWestern Blot: 1.0ug/ml\nImmunohistochemistry: 2-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1014-68B

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Sheep

Form

Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human SNAP23.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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