SNAT1 (amino acids transporter A1, ATA1, NAT2, sodium coupled neutral amino acids transporter 1, SLC38A1)

The sodium-coupled neutral amino acid transporters (SNAT) of the SLC38 gene family include System A subtypes SNAT1, SNAT2 and SNAT4 and System N subtypes SNAT3 and SNAT5. The SLC38 transporters are essential for the uptake of nutrients, energy production, metabolism, detoxification, and the cycling of neurotransmitters. The SNAT1 protein, also designated ATA1 or NAT2 is encoded by the human gene SLC38A1 which maps to chromosome 12q13.11. SNAT1 is responsible for the transport of glutamine, an intermediate in the synthesis of urea, and may be involved in the generation of glutamate in the retina. SNAT1 protein may be detected in some tissues such as heart, brain and placenta and expression levels are enriched in certain neuronal populations within the CNS. SNAT1 is not present in astrocytes.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of SNAT1 in 20ug of lysates from neocortical neurons cultured under amino acid starvation conditions and assayed by Western Blot using goat anti-mouse IgG:HRP as the secondary antibody.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167603

Size

100ug

Applications

ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hatanaka T., et al. ( 2000) Biochim. Biophys. Acta 1467: 1-6.\n2. Wang H., et al. (2000) Biochem. Biophys. Res. Commun. 273: 1175-1179.\n3. Gu S., et al. (2001) J. Biol. Chem. 276: 24137-24144.\n4. Freeman T.L., et al. (2002) Arch. Biochem. Biophys. 400: 215-222.\n5. Palii S.S., et al. (2004) J. Biol. Chem. 279: 3463-3471.\n6. Sidoryk M., et al. (2004) Neuroreport. 15: 575-578.\n Erratum in: 2004. Neuroreport. 15: 533.\n7. Desforges,M., et al. (2006) Am. J. Physiol. Cell.\n Physiol. 290: 305-312.\n

Additional Info

Recognizes rat SNAT1. Species Crossreactivity: Human, Mouse, Rat. Detects ~50kD.