SOCS 1 (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1)

The suppressor of cytokine signaling (SOCS) family of proteins (SOCS1-SOCS7 and CIS) inhibit cytokine production by a classical negative feedback mechanism. These proteins are upregulated in response to cytokine stimulation followed by inhibition of cytokine-inducing signal pathway. Role of each members of the family has been determined by gene knockout experiments (1). SOCS1 also known as JAB and (STAT)-induced STAT inhibitor 1 (SSI-1) is an SH2-domain-containing protein that is induced by and negatively regulates signaling by a number of cytokines including interleukin-4 (IL-4), IL-6, interferon (IFN)-gamma, prolactin, growth hormone, and erythropoietin (1). SOCS1/JAB is rapidly induced by LPS and negatively regulates LPS signaling (2). SOCS1 (-/-) mice are very sensitive to LPS. These mice die prematurely, probably due to uncontrolled IFN-g signaling (3). \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\npositive control:\nHuman Ramos or mouse Raw cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1040-01D

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G chromatography

Isotype

IgG

References

1. Krebs DL, Hilton DJ. Stem Cells 19(5):378-387 (2001). \n\n2. Kinjyo I, Hanada T, Inagaki-Ohara K, Mori H, et al. Immunity 17(5):583-591 (2002). \n\n3. Ilangumaran S, Finan D, La Rose J, Raine J, Silverstein A, De Sepulveda P, Rottapel R. J Immunol 169(9):5010-5020 (2002).

Additional Info

Recognizes human SOCS 1 (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1). Species Crossreactivity: Mouse and human. Species sequence homology: Rat.